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fluorescein isothiocyanate

fluorescein isothiocyanate

9.6
A yellow-green dye that shines under a microscope and is used to mark proteins or antibodies
  • noun
  • /flɔːˈrɛs.iˌin aɪ.soʊˌθaɪ.oʊˈkaɪ.ən.eɪt/
  • Specialized
translation icon : isotiocianato de fluoresceína
  • We used fluorescein isothiocyanate to label the antibodies for the immunofluorescence microscopy study.
  • fluorescein isothiocyanate-labeled

Examples

  • The secondary antibody was a fluorescein isothiocyanate-labeled goat antihuman IgG.

    Academic text (2010)
  • We used FITC (fluorescein isothiocyanate)-avidin D to label DNA strand breaks.

    Academic text (2014)
  • The Fluorescein isothiocyanate (FITC)-conjugated phalloidin (1:1,000) was added to the cells at 4 degrees C overnight.

    Academic text (2018)
  • NMJ changes were decorated with fluorescein isothiocyanate-labeled a-bungarotoxin (Invitrogen) and phalloidin as a control using the manufacturer's recommended protocol.

    Academic text (2011)
  • The fluorescein isothiocyanate dye emitted a bright green glow under the UV light during the experiment.

  • For the analysis, the researchers prepared a sample stained with fluorescein isothiocyanate to visualize protein localization.

Synonyms

fluorescein isocyanate
vsfluorescein isothiocyanate
  • Specialized
7.9

A bright green dye that attaches to antibodies to make proteins glow under a fluorescence microscope

is a yellow green dye used specifically to label antibodies and proteins
fluorochrome
vsfluorescein isothiocyanate
  • Specialized
9.8

A chemical that glows under a microscope to stain cells

is narrower, focusing on labeling antibodies and proteins rather than general staining

Surface Forms

Morphology

fluorescein + isothiocyanate

This is a technical compound name formed by straightforwardly combining 'fluorescein' (a dye that fluoresces) with 'isothiocyanate' (a chemical functional group), so a learner who knows both components can infer it refers to a fluorescent isothiocyanate derivative. However, the exact chemical relation and specific application (e.g., antibody labeling, yellow-green emission) are specialized and not fully predictable for a B1 learner, so the transparency is only partial.

Etymology

Fluorescein isothiocyanate tells a simple story: fluorescein is a yellow-green dye that 'glows' and isothiocyanate is the part that 'sticks' to proteins or antibodies. That's why the name means a glowing tag used to 'label' antibodies so scientists can see them under the microscope.