Skip to main content
transfection

transfection

1 9.5
To put new genes or DNA into living cells for experiments
  • noun
  • /trænsˈfɛkʃən/
  • Specialized
translation icon : transfección
  • In vitro, they can be delivered by transient plasmid transfections or viral transduction for long-term silencing.
  • siRNA transfection
  • transfection of cells
  • hours after transfection

Examples

  • After siRNA transfection overnight, the cells were incubated with shikonin at the indicated dose for subsequent experiments.

    Academic text (2016)
  • Researchers conducted a transfection to introduce new DNA into the cells for their experiment.

  • The expression rate of a target protein is a key metric for transfection efficiency.

    Academic text (2018)
  • The results showed that the coprecipitation of DNA-lipoplexes resulted in the highest transfection efficiency in all groups.

    Academic text (2018)
  • After 24 h, transfection reagent was replaced with normal cell culture media containing ruxolitinib where indicated.

    Academic text (2017)
  • Cells were plated at 80% confluency for transfection experiments.

    Academic text (2018)
  • The results revealed that this liposome had a higher transfection rate in osteoblastic cell lines than in the 294T line.

    Academic text (2018)
  • The optimal balance between transfection rate and cytotoxicity in designing novel nVVs has not yet been achieved.

    Academic text (2018)
  • After transfection for 12 hours, the cells were trypsinized, counted, and resuspended in the serum-free medium.

    Academic text (2014)
  • The sMUA AuPAMAM constructs showed the highest stability, gene transfection efficacy, and a reasonable cytotoxicity profile.

    Academic text (2018)

Synonyms

transformation
vstransfection
  • Specialized
4 6.5

A cell taking in outside DNA and changing its traits

is an experimental method to introduce genetic material into cells
transduction
vstransfection
  • Specialized
9.9

The transfer of genes between bacteria by a virus that carries DNA

is done in labs using direct techniques rather than biological vectors

Surface Forms

transfection singular
transfections plural

Morphology

transfection = infection (semi-transparent) = trans + fect + ion

The prefix 'trans-' adds the meaning of 'across' or 'through', but the specific scientific meaning is not obvious without specialized knowledge.

Etymology

Transfection starts with trans- meaning 'across' and shares the same root as infect, which gives the image of putting something into cells. So transfection means putting foreign DNA or genes 'across into' cells, which is exactly how scientists use the word today.