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lysate

lysate

1 9.1
A liquid that contains parts of cells released when the cells are broken, used in research
  • noun
  • /ˈlaɪseɪt/
  • Specialized
: lisado
  • In the laboratory, we prepared the lysate from the broken cells for analysis.
  • cell lysate

Examples

  • A total of 4 ml of worm lysate was used as a PCR template.

    Academic text (2017)
  • Lysate was serially diluted, plated on agar plates by the track method, and CFU were counted the next day.

    Academic text (2017)
  • Plate lysates were used in all of the experiments described below.

    Academic text (2003)
  • For PCR, 2 L of a 1:400 dilution of lysate was used.

    Academic text (2012)
  • We demonstrate that Beclin 1 is cleaved in brain lysates of human AD patients.

    Academic text (2018)
  • The lysate was saved in fresh Eppendorf tubes, which were subsequently stored at -20°C until further use.

    Academic text (2014)
  • Crude lysates were centrifuged at 12,000 g for 10 min at 4 degrees C to remove cellular debris.

    Academic text (2018)
  • Clarified lysates were quantified with a Pierce BCA protein assay.

    Academic text (2018)
  • We then calculated the amount of TF per cultured islet after quantifying TF in the lysates by ELISA.

    Academic text (2002)
  • To determine whether Beclin 1 cleavage occurs in the context of a neurodegenerative disease, we performed Western blot analysis on the RIPA-soluble fraction of cortical brain lysates from AD patients and healthy controls.

    Academic text (2018)

Surface Forms

lysate singular
lysates plural

Morphology

lysate = lyse (opaque) = lyse + ate

The word is built from the technical verb 'lyse' plus the suffix '-ate', but this relationship is not transparent for most learners because 'lyse' is specialized biological vocabulary.

Etymology

The word lysate comes from the Greek root lys- meaning 'to break', and you can see the same root in analysis, where it means 'breaking down'. The ending -ate means 'a product'. So a lysate is the product you get when cells are broken open and their contents are released.