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Glite Dictionary
to biotinylate

to biotinylate

1 9.8
Attach a biotin molecule to another molecule to make it easy to detect
  • verb
  • /ˌbaɪəˈtɪnɪˌleɪt/
  • Specialized
translation icon : biotinilar
  • To visualize the protein, the lab technician will biotinylate the sample using a biotin reagent.
  • biotinylated anti-mouse IgG
  • biotinylated anti

Examples

  • Samples were then incubated with a biotinylated secondary antibody (XMG1.2) and a streptavidin-HRP for developing.

    Academic text (2017)

  • Then add 100 µl of biotinylated antibody working solution to each well.

    Blog text (22)

  • A biotinylated anti-Abeta primary antibody (3D6) was used for amyloid beta plaque detection, as previously described 41.

    Academic text (2018)

  • The standard immunoperoxidase sequence, using respective biotinylated antisera and the Vector ABC system (Vector Labs, Burlingame, CA), was used in the final staining.

    Academic text (1995)

  • Researchers often biotinylate antibodies to improve their detection in various assays.

  • In cellular studies, scientists may biotinylate proteins to track their interactions with other molecules.

Synonyms

to label
vsto biotinylate
  • Specialized
14 7.9

To add a radioactive marker to a molecule so scientists can track it

attaches a specific small molecule tag for detection rather than isotope marking

How Labeled

to biotinylate
  • Specialized
1 9.8
to label
92 1.0
tag
180 1.0
to mark
192 1.0

Surface Forms

biotinylate infinitive
biotinylated past
biotinylated past participle
biotinylating present participle
biotinylates third person singular

Morphology

biotinylate = biotin (semi-transparent) = biotin + ylate

Formed by adding the specialized chemical suffix '-ylate' to 'biotin' to mean 'attach a biotin group'; the pattern is regular in scientific usage but the suffix is technical and unfamiliar to general learners.

Etymology

Biotinylate comes from biotin, a small vitamin whose first part bio- means 'life', and the chemical ending -ylate, which means 'to add' or 'attach' a group. So, to biotinylate is to 'attach' a biotin tag to a molecule to make it easier to find or see.