cDNA
2
8.4
- noun
- /siːˌdiːˈɛnˈeɪ/
- Specialized
:
cADN
- Total mRNA was extracted from the cells, and cDNA was synthesized by reverse transcription. The primer used was Olido-dT.
- cDNA library
- cDNA sequence
- cDNA clone
You do second strand synthesis, now you have double stranded cDNA fragments.
- You do second strand synthesis, now you have double stranded cDNA fragments.
- OK Any other questions about the cDNA library?
Examples
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The two most common technologies are cDNA microarrays and oligonucleotide expression arrays.
Academic text (2010) -
Each participant's sample of cDNA was analyzed by two independent PCR runs.
Academic text (1996) -
OK Any other questions about the cDNA library?
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A partial sequence of a cDNA molecule, usually unique and identifying a specific gene.
Academic text (2008) -
The isolated full-length cDNA sequences were compared with human genomic sequences.
Academic text (2003) -
The genomic structure of SYCP3 was determined by comparison of the cDNA sequence with the human genomic sequence database.
Academic text (2003) -
To validate the intron-exon structure predicted by the computer software, full-length cDNA was cloned for the candidate gene.
Academic text (2018) -
For mutation analysis, the full-length cDNA sequences were compared with human genomic sequences, and all exon-intron borders were identified.
Academic text (2003) -
Total RNA was reverse transcribed to cDNA using a High-Capacity RNA-to-cDNA Kit (Applied Biosystems, Foster City, CA, USA).
Academic text (2014) -
To study gene expression, researchers synthesized cDNA from the mRNA of the sample cells.
Synonyms
A single-stranded DNA made from messenger RNA that shows which genes are active
Surface Forms
Morphology
cDNA = DNA (opaque) = c + D + N + A
The connection to 'DNA' is visible, but the initial 'c' is specialized scientific shorthand for 'complementary', so the full sense is not inferable for most learners.
Etymology
The word cDNA is short for complementary DNA, where 'complementary' means 'matching' or 'fitting together'. So cDNA is DNA made to match an RNA copy of a gene, which is why scientists make it when they want a DNA version of the gene's message.