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amplicon

amplicon

1 8.8
A piece of DNA that scientists copy many times in a lab for tests
  • noun
  • /ˈæmplɪˌkɒn/
  • Specialized
translation icon : amplicón
  • The amplicons were resolved by agarose gel electrophoresis at 200 volts for 40 minutes.
  • mega-amplicon
  • PCR amplicons
  • amplicon sequencing

Examples

  • Evaluation of Nanomicroarray Assay by using PCR Mega-amplicons.

    Academic text (2015)
  • The amplicon (GenBank accession no. JN800509) was sequenced by using standard primers and protocols (10).

    Academic text (2012)
  • Several internal primers were designed to derive the complete sequences of the PCR amplicons.

    Academic text (2012)
  • To accompany each amplicon library, we normalize bacterial cell counts with qPCR and rRNA gene copy number.

    Academic text (2018)
  • The amplicon libraries were sequenced on a HiSeq 2500 platform (Illumina, San Diego, CA, USA).

    Academic text (2018)
  • The sequences of the corresponding amplicons were determined and deposited into GenBank under accession nos. KC592396-KC592411.

    Academic text (2013)
  • Nine of the 12 sequenced amplicons showed high identity among themselves and with the APMV sequence (GenBank accession no. HQ336222).

    Academic text (2014)
  • The amplicons were separated on 10% polyacrylamide gels and visualized after staining with ethidium bromide.

    Academic text (2013)
  • The mega-amplicons of the A/Vietnam/1204/2004 (H5N1) strain yielded 8 contiguous sequences supported by 90,962 reads.

    Academic text (2015)
  • The researcher analyzed the amplicon sizes to ensure the accuracy of the DNA test results.

How Specific

locus
  • Specialized
3 9.0
amplicon
  • Specialized
1 8.8
target area
  • Specialized
1 6.1

Surface Forms

amplicon singular
amplicons plural

Morphology

amplicon = amplify (semi-transparent) = amplic + on

Formally built from a combining form meaning 'to amplify' plus the noun-forming biological suffix '-on'; the link to 'amplify' is clear but the specific technical sense ('an amplified DNA fragment') is specialized.

Etymology

The word amplicon comes from the same root as amplify and ample, which mean 'large' or 'make bigger'. In biology, an amplicon is a piece of DNA that has been 'made bigger' by copying many times, so the origin helps explain its modern meaning.